• 1. NCBI Probe Database
• NCBI. Probe Database is a public registry of nucleic acid reagents designed for use in a wide variety of biomedical research applications, together with information on reagent distributors, probe effectiveness, and computed sequence similarities.

• 2. ProbeWiz server
• Center for Biological Sequence Analysis. Design PCR primers for amplifying probes for cDNA arrays, with the goal of reducing or eliminating cross-hybridizations

• 3. PUNS: Primer-UniGene Selectivity Testing
• Compares primer sequences against the both the genome and transcriptome to assess the potential for multiple amplicons.

• 4. Quantitative PCR Primer Database - QPPD
• NIH, NCI. The Quantitative PCR Primer Database (QPPD) provides information about primers and probes that can be used to quantitate human and mouse mRNA by reverse transcription polymerase chain reaction (RT–PCR) assays.

• 5. RTPrimerDB: the real-time PCR primer and probe database
• Ghent University. RTPrimerDB is a public database for primer and probe sequences used in real-time PCR assays employing popular chemistries (SYBR Green I, Taqman, Hybridisation Probes, Molecular Beacon) to prevent time-consuming primer design and experimental optimisation, and to introduce a certain level of uniformity and standardisation among different laboratories.

• 6. SNP Annotation Platform - SNAP
• Snap is a sequence analysis tool providing analysis of human genes and their variations. For each gene, a gene-gene relationship network can be generated based on protein-protein interaction data, metabolic pathway connections and extended through phylogenetic relations. Snap provides tools for designing sequence primers and evaluating RNA splicing effects of single SNPs